Agromonas: a rapid disease assay for<i>Pseudomonas syringae</i>growth in agroinfiltrated leaves

The lengthy process to generate transformed plants is a limitation in current research on the interactions of model plant pathogen Pseudomonas syringae with hosts. Here we present an easy method called agromonas, where quantify P. growth agroinfiltrated leaves Nicotiana benthamiana using cocktail antibiotics select plates. As proof concept, demonstrate that transient expression PAMP receptors reduces bacterial growth, and depletion host immune gene type-III effector increase agromonas assays. We show can rapidly achieve structure?function analysis components test function hydrolases. easy, fast robust for routine disease assays various strains without transforming or bacteria. assay offers reliable approach further comprehensive immunity.